HIGH SENSITIVITY DOUBLE COLOR MICROARRAY SLIDES
AmpliSlide™ technology from Genewave delivers the highest sensitivity for fluorescence based microarray applications. AmpliSlide™ incorporates the combination of a unique fluorescence-amplifying thin film optical coating and a high quality surface chemistry. Double Color AmpliSlide™ has been tailored to provide a balanced fluorescence enhancement for Cy®3/Cy5 or analogous dyes. Slides are available either with Aminosilane or Epoxysilane chemistry.
Associated products
More information
Benefits
- Significant improvement of microarray sensitivity
- Reliable detection of low-expressed genes
- Reduction of required sample amounts such as biopsies
- Reduction of the number of cell passaging steps
- Savings on costly reagents such as dye labeling amplification kits
- Faster microarray imaging
Key Features
- Typically 16-fold signal fluorescence enhancement*
- Excellent probe binding efficiency
- Preservation of channel ratios
- Compatible with common microarray equipment and protocols
- Chemically robust reflective coating
- Easy identification of reflective side
Applications
AmpliSlide™ A (Aminosilane chemistry) are recommended for cDNA, PCR products and unmodified oligonucleotides.
AmpliSlide™ E (Epoxysilane chemistry) are recommended for modified oligonucleotides, peptides and proteins.
Other chemistries are available: please contact us.
Significant signal enhancement
Data kindly provided by Dr. Nicolas Maunoury from CGM (Centre de Génétique Moléculaire, CNRS) - GODMAP, Gif-sur-Yvette, France.
Fig.1 Comparison between identical high density DNA arrays spotted on AmpliSlide™ A and on a leading competitor's aminosilane slide. These images show a very significant enhancement of the signal intensity on AmpliSlide™. Both slides were spotted, hybridized and scanned under the same conditions (including laser power and PMT gain value).
Highest signal-to-noise ratios
Data kindly provided by Dr. Olivier Delattre from Institut Curie, Paris, France.
These graphs show the data obtained for 10k high-density CGH-arrays arrayed and hybridized simultaneously on AmpliSlideTM and leading conventional glass aminosilane slides. The best scanning conditions were used for each slide in order to obtain maximal non-saturated signals. SNR=(S-B)/SD(B).
Fig.2 AmpliSlide™ A slides exhibit a 4-fold signal-to-noise ratio (SNR) enhancement when compared to leading competitor's glass aminosilane slides. The fluorescence enhancement gives rise to a clear shift of SNR distribution to high values. SNR=(S-B)/SD(B).
Fig.3 SNR enhancement results in a significant reduction of the number of unreliable spots (with SNR<3), which drops from 20% to 4% with AmpliSlide™ A substrate. The circles' surfaces are proportional to the total number of unreliable spots for each slide which are indicated near the circles.
Specifications
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Slide size | 75 x 25 mm (+/- 0.2 mm) |
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Chemistry | Aminosilane or Epoxysilane (compatible with conventional buffers and protocols) |
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Experiment model | Double (balanced channels) |
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Dye compatibility | Dyes emitting around 570 and 670 nm: Cy®3/Cy5, Alexa®546/Alexa647, DY®-548/DY-648 |
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Signal enhancement | 10 to 20 fold* |
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Sensitivity enhancement | 4-fold* |
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Scanner compatibility | Any top-reading confocal laser scanners or CCD-based readers** |
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Barcode | With or without |
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Printing area | 63 x 23 mm (with barcode) or 70 x 23 mm (without barcode) |
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Packaging | Vacuum sealed, in packs of 5 or 25 slides |
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Shelf life | 9 months (in original packaging) |
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Storage conditions | Room temperature |
* Typical values when compared with leading competitor's conventional glass aminosilane and epoxysilane slides. Exact values depend on the optical architecture of the scanner and reference slide.
** Note: scanners reading slides only from the back side are not compatible with reflective slides.
